Coding

Part:BBa_K648028

Designed by: James Coletta, Anisha Katyal, Kristen Salavo, Lauren Rossi   Group: iGEM11_Penn_State   (2011-07-25)


Cro, Lamda Repressor which activates the lytic cycle

Cro is produced by the pr promoter in the lambda phage system. Normally C1 is repressing the pr promoter. However, when the lytic switch is thrown and RecA cleaves the C1 dimer the pr promoter is unrepressed. This results in the production of cro which in turn represses the prm promoter which halts the transcription of C1 repressor. In our project we used Cro to assemble our lambda system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None